Inhibition of penicillin biosynthetic enzymes by halogen derivatives of phenylacetic acid |
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Authors: | Honorina Martínez-Blanco Angel Reglero Dr José M Luengo |
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Institution: | (1) Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de León, 24007 León, Spain |
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Abstract: | Summary The effect of phenylacetic acid (PAA) and several analogs on the activity of isopenicillin N synthase (IPNS) and acyl-CoA: 6-APA acyltransferase (AT) fromPenicillium chrysogenum Wis 54-1255 has been tested. Whereas the substitution on the ring of a hydrogen atom by hydroxy-, methyl- or methoxy- groups did not cause any effect, the presence of halogens (Cl or Br) at positions 3 and/or 4 of PAA strongly inhibited these two enzymes. The replacement of hydrogen atoms by fluorine in certain positions also caused inhibition, but to a lesser extent. |
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Keywords: | Penicillin Halogenated phenylacetic acid Antibiotic biosynthesis |
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