Regulation of protein kinase FA (a transmembrane signal of insulin and epidermal growth factor) in the brain |
| |
Authors: | S D Yang J S Yu H C Chen |
| |
Institution: | Institute of Molecular Cell Biology, Chang Gung Medical College, Tao-Yuan, Taiwan, Republic of China. |
| |
Abstract: | Two forms of type-1 protein phosphatase activating factor (FA) termed FA1 and FA2 have been identified in plasma membranes of pig brain. FA1 is spontaneously active and trypsin-labile whereas FA2 is inactive and trypsin-resistant. Phospholipid reconstitution studies further indicate that the FA activity in the neutral phospholipids-reconstituted complex is spontaneously active and trypsin-labile whereas the FA activity in the acidic phospholipids-reconstituted complex is trypsin-resistant and inactive. The results indicate that inactive FA2 may have its catalytic domain interacted with negatively-charged phospholipids in brain membranes. This provides initial evidence for the regulation of protein kinase FA (a transmembrane signal of insulin and epidermal growth factor) in the central nervous system. |
| |
Keywords: | |
|
|