The use of time-resolved fluorescence imaging in the study of protein kinase C localisation in cells |
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| Authors: | Email author" target="_blank">Christopher?D?StubbsEmail author Stanley?W?Botchway Simon?J?Slater Anthony?W?Parker |
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| Institution: | (1) Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA;(2) The Central Laser Facility, Rutherford Appleton Laboratory, CCLRC, Chilton, OX11 OQX, UK |
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| Abstract: | Background Two-photon-excitation fluorescence lifetime imaging (2P-FLIM) was used to investigate the association of protein kinase C
alpha (PKCα) with caveolin in CHO cells. PKCα is found widely in the cytoplasm and nucleus in most cells. Upon activation,
as a result of increased intracellular Ca2+ and production of DAG, through G-protein coupled-phospholipase C signalling, PKC translocates to a variety of regions in
the cell where it phosphorylates and interacts with many signalling pathways. Due to its wide distribution, discerning a particular
interaction from others within the cell is extremely difficult |
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