Study of the thermokinetic properties of copper(II) on Escherichia coli growth |
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| Authors: | Jun Yao Yi Liu Peng Liu Huigang Liang Ming Sun Songsheng Qu Ziniu Yu |
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| Affiliation: | (1) Key Laboratory of Agricultural Microbiology of Chinese Agriculture Ministry, Huazhong Agricultural University, 430070 Wuhan, People’s Republic of China;(2) Department of Chemistry, College of Chemistry and Molecular Science, Wuhan University, 430072 Wuhan, People’s Republic of China;(3) College of Life Science and Chemistry, Jishou University, Jishou, 416000 Hunan, People’s Republic of China |
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| Abstract: | By using an LKB2277 Bioactivity Monitor, stop-flow mode, the power-time curves of Escherichia coli at 37°C affected by Cu(II) were determined. Some parameters, such as growth rate constants k, inhibitory ratio I, the heat output Qlog in the log phase, and the generation times G were obtained. According to these parameters, we found that a low concentration of Cu(II) (0–20 μg/mL) had an promoting action on the growth of E. coli, but a high concentration of Cu(II) (40–100 μg/mL) had an inhibitory action. The toxicity of Cu(II) can also be expressed as the half-inhibitory concentration IC50; the value is 69.7 μg/mL. The assay is quantitative, inexpensive, and versatile. |
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| Keywords: | Microcalorimetry thermokinetics Escherichia coli Cu(II) growth metabolism |
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