| 七种我国昆虫病原线虫共生菌的分离与鉴定 |
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| 引用本文： | 杨秀芬,刘 峥,张 冉,杨怀文,袁京京,简 恒. 七种我国昆虫病原线虫共生菌的分离与鉴定[J]. 微生物学通报, 2008, 35(2): 0225-0229 |
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| 作者姓名： | 杨秀芬 刘 峥 张 冉 杨怀文 袁京京 简 恒 |
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| 作者单位： | 中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京,100081 |
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| 基金项目： | 农业微生物菌种资源整理、整合及共享试点项目 |
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| 摘 要： | 昆虫病原线虫共生菌是一类具有广阔发展前景的生物防治资源,由于这类菌的分类鉴定工作起步晚,存在分类混乱和不系统的现象,在本土资源十分丰富的我国尤为突出.本文对本实验室分离的7个共生菌菌株进行了分类描述,建立了以生理形态,生化特征为分类基础,结合16 SrDNA序列分析的有效鉴定方法.
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| 关 键 词： | 昆虫病原线虫共生细菌 致病杆菌属 光杆状菌属 16S rDNA 分类鉴定 昆虫病原线虫共生菌 分离与鉴定 Entomopathogenic Nematodes Local Bacteria Identification 鉴定方法 序列分析 结合 分类基 生化特征 形态 生理 分类描述 菌株 实验室 本土资源 现象 系统 存在 |
| 修稿时间： | 2007-08-12 |
Isolation and Identification of Seven Symbiotic Bacteria from Local Entomopathogenic Nematodes |
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| YANG Xiu-Fen,LIU Zheng,ZHANG Ran,YANG Huai-Wen,YUAN Jing-Jing and JIAN Heng. Isolation and Identification of Seven Symbiotic Bacteria from Local Entomopathogenic Nematodes[J]. Microbiology China, 2008, 35(2): 0225-0229 |
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| Authors: | YANG Xiu-Fen LIU Zheng ZHANG Ran YANG Huai-Wen YUAN Jing-Jing JIAN Heng |
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| Affiliation: | The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academic of Agricultural Science, Beijing 100081;The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academic of Agricultural Science, Beijing 100081;The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academic of Agricultural Science, Beijing 100081;The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academic of Agricultural Science, Beijing 100081;The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academic of Agricultural Science, Beijing 100081;The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academic of Agricultural Science, Beijing 100081 |
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| Abstract: | The symbiotic bacterium exists in the intestines of entomopathogenic nematodes and is a potential biological agent. Systematic classification of these bacteria is scarce in China. In this paper, seven strains of symbiotic bacteria from local entomopathogenic nematodes were identified by both observation of morphology, physiological, biochemical characteristics and sequence analysis of 16S rDNA fragments. |
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| Keywords: | Symbiotic bacterium of entomopathogenic nematode Xenorhabdus Photorhabdus 16S rDNA Identification |
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