Long-chain fatty acids and ethanol affect the properties of membranes inDrosophila melanogaster larvae |
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Authors: | Robert R. Miller Jr. Amos O. Dare Marcie L. Moore Robert G. Kooser Billy W. Geer |
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Affiliation: | (1) Department of Biology, Knox College, 61401-4999 Galesburg, Illinois;(2) Department of Chemistry, Knox College, 61401-4999 Galesburg, Illinois;(3) Present address: Biology Department, Grand View College, 50316-1599 Des Moines, Iowa;(4) School of Medicine, Yale University, 06516 New Haven, Connecticut |
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Abstract: | The larval fatty acid composition of neutral lipids and membrane lipids was determined in three ethanol-tolerant strains ofDrosophila melanogaster. Dietary ethanol promoted a decrease in long-chain fatty acids in neutral lipids along with enhanced alcohol dehydrogenase (EC 1.1.1.1) activity in all of the strains. Dietary ethanol also increased the incorporation of14C-ethanol into fatty acid ethyl esters (FAEE) by two- to threefold and decreased the incorporation of14C-ethanol into free fatty acids (FFA). When cultured on sterile, defined media with stearic acid at 0 to 5 mM, stearic acid decreased ADH activity up to 33%. In strains not selected for superior tolerance to ethanol, dietary ethanol promoted a loss of long-chain fatty acids in membrane lipids. The loss of long-chain fatty acids in membranes was strongly correlated with increased fluidity in hydrophobic domains of mitochondrial membranes as determined by electron spin resonance and correlated with a loss of ethanol tolerance. In the ethanol-tolerant E2 strain, which had been exposed to ethanol for many generations, dietary ethanol failed to promote a loss of long-chain fatty acids in membrane lipids. We are grateful for the support of National Institutes of Health Grant AA06702 (B.W.G.) and National Science Foundation Grant CHE-891987 (R.G.K.). |
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Keywords: | ethanol-induced ethanol tolerance long-chain fatty acids alcohol dehydrogenase membrane fluidity fatty acid ethyl esters |
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