The influence of differentiation-inducing agents on plasma membrane surface characteristics of THP-1 cells |
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Authors: | S V Zubova D S Kabanov A Yu Ivanov E V Voloshina I I Proskuryakov I B Klenina I R Prokhorenko |
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Institution: | 1.Institute of Basic Biological Problems,Russian Academy of Sciences,Pushchino, Moscow oblast,Russia;2.Institute of Cell Biophysics,Russian Academy of Sciences,Pushchino, Moscow oblast,Russia |
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Abstract: | Undifferentiated THP-1 cells from Cell Culture Collection of the Institute of Cytology, RAS (St. Petersburg), are characterized
by weak expression of Toll-like receptor-4 (TLR4) on the cell surface (up to 2%) and by almost undetectable expression of
CD14 and CD11b receptors. Differentiation agent phorbol-12-myristate-13-acetate independently of its concentration (2 × 10−7 M or 10−8 M) and incubation time (24 or 48 h) did not initiate CD11b surface expression and did not change the parameter Sapp (0.605 ± 0.005 at 37°C) reflecting the cell membrane viscosity. Differentiation of THP-1 cells induced by another differentiation
agent, 1α,25-dihydroxyvitamin D3, caused expression of CD14 (up to 70–80%) and CD11b (up to 15–20%) receptors, again without changes in plasma membrane viscosity.
The rate constants of the reduction of 5- and 16-doxyl-stearic acids by THP-1 cells were in the range of 6–8 × 10−3 s−1 at 37°C. During cell differentiation significant changes in cell electrophoretic mobility (EM, μm s−1 V−1 cm) were observed. Mean value of EM for undifferentiated THP-1 cells was −1.332 ± 0.011, whereas for phorbol-12-myristate-13-acetate-
and 1α,25-dihydroxyvitamin D3-treated cells it was −1.432 ± 0.030 and −1.212 ± 0.016, respectively. |
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