The localization of tRNAAsp2 genes from Drosophila melanogaster by "in situ" hybridization. |
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Authors: | Thomas Schmidt Albert H Egg and Eric Kubli |
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Institution: | (1) Zoologisch-Vergl. Anatomisches Institut der Universität Zürich, Künstlergasse 16, CH-8006 Zürich, Switzerland |
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Abstract: | Transfer RNAAsp2delta was isolated from Drosophila melanogaster by affinity chromatography on concanavalin A-Sepharose. The tRNA was iodinated "in vitro" with Na 125I] and hybridized "in situ" to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNAAsp2 to the left arm of the second chromosome in the regions 29 D and E. |
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