|
|||||
|
|
Production of monoclonal antibodies and development of an ELISA for solamargine |
|
| Authors: | Masato Ishiyama Yukihiro Shoyama Hiroki Murakami Hideo Shinohara |
| Affiliation: | (1) Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812 Fukuoka, Japan;(2) Graduate School of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812 Fukuoka, Japan;(3) Present address: Central Research Laboratories, Kissei Pharmaceutical Co. Ltd., 399-65 Matsumoto-city, Nagano Prefecture, Japan |
| Abstract: | The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibodies against solamargine was produced by fusing splenocytes immunized with a solamargine-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. Extensive cross-reaction of anti-solamargine antibodies against solasonine appeared. Aglycone of solamargine, solasodine cross-reacted with anti-solamargine antibodies resulting in a 43.8% cross-reaction. Insignificant cross-reaction appeared with tomatine (2.06%). The full measuring range of the assay extends from 57.5 pmol ml–1 to 11.5 nmol ml–1 of solamargine. |
| Keywords: | steroidal alkaloid Solanum khasianum solamargine ELISA monoclonal antibody qualitative analysis mass spectrometry |
| 本文献已被 PubMed SpringerLink 等数据库收录！ | |