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Genotype-independent transformation of lettuce using Agrobacterium tumefaciens
Authors:Curtis  IS; Power  JB; Blackhall  NW; de Laat  AMM; Davey  MR
Abstract:The genetic manipulation of lettuce (Lactuca sativa) necessitatesa reliable and efficient, genotype-independent method of transformation.Thirteen lettuce cultivars have been assessed for their amenabilityto Agrobacterrum-mediated gene transfer linked to their tissueculture responsiveness, including callus induction and shootregeneration. A reliable protocol has been developed for theroutine production of transgenic plants for all 13 cultivarsinvestigated. Key words: Agrobacterium-mediated transformation, Lactuca sativa genotypes, lettuce
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