激光诱变选育高产PUFAs真菌菌株的研究

采用PIV400型Nd：YAG激光器，对少见报道的产PUFAs丝状真菌菌株Monoblepharis polymorpha Comu进行激光诱变育种。在激光照射剂量：波长532nm，功率50mW，时间10min下，孢子致死率为75％-80％。突变株中w-3系列PUFAs正突变率大于80％，w-3系列突变株FPAm59传代10代以上性能稳定，在土豆基质上摇瓶培养6d后，生物量达到8g/L，产油率53．4％，GLA343．1mg／L、从1209．0mg／L、EPA125．1mg／L、DHA119．4mg／L；分别比对照菌株提高了2．5倍、9．7倍、18．0倍、6．6倍、10．3倍、3．8倍。结果表明，激光诱变育种是获得PUFAs高产菌株尤其是生产w-3系列PUFAs菌株的有效方法。

Study on Screening of Fungi Strain with Highly Content PUFAs by Laser Mutation Method

In order to obtain mutant fungi strain with highly content PUFAs. The fungi spore in the plate were radiated at wavelength 532 nm for 10 minutes using Nd:YAG laser,the lethality ratio of spores is approximately 75%～80%.The mutation rate of series ω-3 is above 80%.the mutation strain FPAm59 can retain its stability during more than 10 passages.After six days culture,the biomass of FPAm59 is up to 8 g/L and 2.5 times of the control strains.The lipid component of the biomass is 53.4% and (9.7) times of control strains.The quantity of the GLA,AA,EPA,and DHA in the strain lipid is 343.1mg/L, 1 209.0 mg/L,125.1mg/L,119.4mg/L and 18.0 times,6.6 times,10.3 times,and 3.8 times of control strains lipid.Therefore,laser mutation to fungi is an effective method to obtain the high quantity of PUFAs especially to the ω-3 series.