Analysis of metabolic profiles of prostaglandins in urine using a lipophilic anion exchanger |
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Authors: | B. Alm ,G. Hansson |
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Affiliation: | Department of Chemistry, Karolinska Institutet, S-104 01 Stockholm, Sweden |
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Abstract: | A method is described for the fractionation of prostaglandins and their metabolites in urine. Following acidification and extraction on Amberlite XAD-2, samples were separated by chromatography on the lipophilic anion exchanger diethyl-aminohydroxypropyl Sephadex LH-20 into fractions containing neutral compounds, monocarboxylic, dicarboxylic and polycarboxylic acids. The compounds in resulting fractions were further separated by reversed phase partition chromatography. As an application, the metabolic profiles in urine of [9β-3H]-labeled prostaglandin F2α1 and prostaglandin analogs 15-methyl-PGF2α and 16,16-dimethyl-PGF2α were investigated in the cynomolgus monkey. It was demonstrated that the resolution of individual prostaglandin metabolites by reversed phase partition chromatography was considerably simplified by initial group separation on the anion exchanger, and several metabolites were much purified. A glucuronic acid conjugate of the main metabolite of 15-methyl-PGF2α (dinor-15-methyl-PGF2α) was tentatively identified using computerized gas chromatography - mass spectrometry. |
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Keywords: | TMS, trimethylsilyl RPPC, reversed phase partition chromatography GC/MS, gas chromatography - mass spectrometry |
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