In vitro ectomycorrhiza formation between Abies firma and Pisolithus tinctorius |
| |
| Authors: | L-M Vaario Megumi Tanaka Yuji Ide Warwick M Gill Kazuo Suzuki |
| |
| Institution: | (1) Laboratory of Forest Botany, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan e-mail: lumin@uf.a.u-tokyo.ac.jp Fax: +81-3-5841-7554, JP;(2) Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan, JP;(3) The University Forests, Faculty of Agriculture, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan, JP |
| |
| Abstract: | The first in vitro aseptic synthesis of Abies firma Sieb. et Zucc. with Pisolithus tinctorius (Pers.) Coker & Couch is reported. Techniques were improved for the aseptic synthesis of ectomycorrhizas of A. firma, a slow-growing species in vitro, and Pisolithus tinctorius using a novel culture medium and both sterilized and re-rooted seedlings. After 2–3 months incubation, ectomycorrhizas were
formed by both methods. The mycorrhizas possessed a mantle and a highly branched nonseptate Hartig net mycelium colonizing
the intercellular spaces within the host cortex, features characteristic of ectomycorrhizas. These techniques will prove useful
for addressing physiological and biochemical questions on the interactions of microbes with roots of whole plants.
Accepted: 29 June 1999 |
| |
| Keywords: | Japanese fir Momi fir Ectomycorrhizas In vitro culture Kotsubutake |
| 本文献已被 SpringerLink 等数据库收录！ |
|
