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Arachidonic acid inhibits 5-lipoxygenase in human T cells
Authors:D Atluru  E A Lianos  J S Goodwin
Institution:1. Department of Food and Nutrition, College of Human Ecology, Seoul National University, Seoul, Korea;2. Department of Food and Nutrition, College of Human Ecology, Chungbuk National University, Cheongju, Korea;3. Research Institute of Human Ecology, Seoul National University, Seoul, Korea;1. Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University Medical Center Groningen, Hanzeplein 1, 9700 RB Groningen, The Netherlands;2. Laboratory of Food Chemistry, Wageningen University, PO Box 17, 6700 AA Wageningen, The Netherlands;3. DSM Biotechnology Center, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands;4. Nuscience Group Headquarters, Booiebos 5, 9031 Ghent (Drongen), Belgium;5. Agrifirm Innovation Center, Landgoedlaan 20, P.O. Box 20018, 7302 HA Apeldoorn, The Netherlands;6. Animal Nutrition Group, Wageningen University, P.O. Box 338, 6700 AH Wageningen, The Netherlands;7. Department of Obstetrics and Gynaecology, University of Groningen, and University Medical Center Groningen, Hanzeplein 1, 9700 RB Groningen, The Netherlands
Abstract:The data on whether T cells produce leukotrienes or other 5-lipoxygenase metabolites of arachidonic acid is conflicting. We report that exogenous arachidonic acid added to phytohemagglutin-stimulated human T cells profoundly inhibits leukotriene B4 production, with 90% inhibition caused by 10(-6) M arachidonic acid. The 12- and 15-lipoxygenase pathways were also inhibited by arachidonic acid. Recent reports that human T cells produce no 5-lipoxygenase metabolites of arachidonic acid might be explained by the fact that the studies used greater than or equal to 10(-5)M arachidonic acid in the incubation media.
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