首页 | 本学科首页   官方微博 | 高级检索  
     


Insertion element IS1 can generate a 10-base pair target duplication
Authors:S Iida  R Hiestand-Nauer
Affiliation:1. Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA;2. Center for Plant Molecular Biology, University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany;1. Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland;2. Department of Biophysics and Morphogenesis of Plants, University of Silesia in Katowice, 40-032 Katowice, Poland;1. State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and National Center for Plant Gene Research, Beijing 100101, China;2. College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;3. Beijing International Center for Mathematical Research, Peking University, Beijing 100871, China;4. Department of Biochemistry, Okayama University of Science, Okayama 700-0005, Japan;5. Zhou Pei-Yuan Center for Applied Mathematics, Tsinghua University, Beijing 100084, China;6. Center for Quantitative Biology, Peking University, Beijing 100871, China;1. Japan Atomic Energy Agency (JAEA), Collaborative Laboratories for Advanced Decommissioning Science (CLADS), 4002 Naritacho, Oarai, Ibaraki Pref., 311-1393, Japan;2. Tokyo Electric Power Company Holdings, Inc, 1-1-3 Uchisaiwai-cho, Chiyoda-ku, Tokyo 100-8560, Japan;1. Khalifa Centre for Genetic Engineering and Biotechnology, United Arab Emirates University, Al Ain, 15551, United Arab Emirates;2. Biology Centre, Czech Academy of Sciences, Institute of Plant Molecular Biology, Branišovská 31, 370 05, České Budějovice, Czech Republic;3. Hop Research Institute, Co. Ltd, Kadaňská 2525, 438 46, Žatec, Czech Republic;4. Institute of Botany, Technical University Dresden, Dresden, Germany
Abstract:Transposable element IS1 is known to generate mainly 9-bp and occasionally 8-bp target duplications upon transposition. We have isolated a plasmid pBR322 derivative having IS1 inserted into a site between the promoter and the structural gene for tetracycline resistance. DNA sequence analysis revealed that integration of this IS1 resulted in a 10-bp target duplication.
Keywords:
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号