Comparison of cerebral NADH and cytochrome aa3 redox shifts during anoxia or hemorrhagic hypotension.

The reduction-oxidation reactions of NADH and cytochrome $\text{aa}{}_3$ to incipient oxygen insufficiency caused by nitrogen ventilation or hemorrhagic hypotension were examined in the exposed cerebral cortex of the cat. A comparison of the onset of redox changes with each procedure shows that cytochrome $\text{aa}{}_3$ reduction precedes the reduction of mitochondrial NAD. This constitutes evidence that, in the living brain, NADH maintains its resting oxidation state at lower cellular oxygen tensions than cytochrome $\text{aa}{}_3$ does, consistent with the differences in oxygen affinity these respiratory chain components exhibit during oxygen titration $\text{in vitro}$.