Effect of altered sterol levels on the transport of amino acids and membrane structure ofMicrosporum gypseum |
| |
Authors: | Seema Vaidya Geeta Bharti Rakesh Pandey G. K. Khuller |
| |
Affiliation: | (1) Department of Biochemistry, Postgraduate Institute of Medical Education and Research, 160 012 Chandigarh, India |
| |
Abstract: | Ergosterol and cholesterol supplementation resulted in a significant increase (1·5-fold) in the sterol content while phospholipid remained unaffected inMicrosporum gypseum. The levels of phosphatidylethanolamine and phosphatidylcholine increased in ergosterol supplemented cells. However, a decrease in phosphatidylcholine and an increase in phosphatidylethanolamine was observed in cholesterol grown cells. The ratio of unsaturated to saturated fatty acids decreased on ergosterol/cholesterol supplementation. The uptake of amino acids (lysine, glycine and aspartic acid) decreased in sterol supplemented cells. Studies with fluorescent probe l-anilinonaphthalene-8-sulfonate showed structural changes in membrane organisation as evident by increased number of binding sites in such cells. |
| |
Keywords: | Ergosterol cholesterol phospholipids fatty acids amino acids transport Microsporum gypseum |
本文献已被 SpringerLink 等数据库收录! |