Effect of altered sterol levels on the transport of amino acids and membrane structure ofMicrosporum gypseum |
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Authors: | Seema Vaidya Geeta Bharti Rakesh Pandey G K Khuller |
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Institution: | (1) Department of Biochemistry, Postgraduate Institute of Medical Education and Research, 160 012 Chandigarh, India |
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Abstract: | Ergosterol and cholesterol supplementation resulted in a significant increase (1·5-fold) in the sterol content while phospholipid
remained unaffected inMicrosporum gypseum. The levels of phosphatidylethanolamine and phosphatidylcholine increased in ergosterol supplemented cells. However, a decrease
in phosphatidylcholine and an increase in phosphatidylethanolamine was observed in cholesterol grown cells. The ratio of unsaturated
to saturated fatty acids decreased on ergosterol/cholesterol supplementation. The uptake of amino acids (lysine, glycine and
aspartic acid) decreased in sterol supplemented cells. Studies with fluorescent probe l-anilinonaphthalene-8-sulfonate showed
structural changes in membrane organisation as evident by increased number of binding sites in such cells. |
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Keywords: | Ergosterol cholesterol phospholipids fatty acids amino acids transport Microsporum gypseum |
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