Spectrophotometric determination of myoglobin in cardiac and skeletal muscle: separation from hemoglobin by subunit-exchange chromatography. |
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| Authors: | S Schuder J B Wittenberg B Haseltine B A Wittenberg |
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| Institution: | Department of Physiology, Albert Einstein College of Medicine, New York, New York 10461 USA |
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| Abstract: | Myoglobin is extracted from muscle and separated from blood hemoglobin by subunit-exchange chromatography on a column of Sepharose 4B to which hemoglobin α-β subunits are linked covalently. Hemoglobin is retained on the column. Myoglobin in the effluent is determined spectrophotometrically as ferrous myoglobin or as carbon monoxide ferrous myoglobin. The method is applicable to cardiac, smooth, or skeletal muscle from mammals, reptiles, birds, and teleost fish, but failed with the one amphibian and the one shark tested. |
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| Keywords: | To whom reprint requests should be addressed |
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