New inhibitors of VEGFR-2 targeting the extracellular domain dimerization process |
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Authors: | Elgamacy Mohammad Amin Shalaby Raed Ahmed Elkodsh Ahmad Tawfik Kamel Amr Fawzy Elsayed Mohamed Saad Abdullah Abou-El-Ella Dalal Abd El Rahman |
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Institution: | 1Faculty of pharmacy, Ain shams university; African union organization st, Abbasia, Cairo, Egypt;2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ain Shams University |
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Abstract: | We are reporting the discovery of small molecule inhibitors for vascular endothelial growth factor receptor type 2 (VEGFR-2) extracellular domain. The VEGFR-2 extracellular domain is responsible for the homo-dimerization process, which has been recently reported as a main step in VEGFR signal transduction cascade. This cascade is essential for the vascularization and survival of most types of cancers. Two main design strategies were used; Molecular docking-based Virtual Screening and Fragment Based Design (FBD). A virtual library of drug like compounds was screened using a cascade of docking techniques in order to discover an inhibitor that binds to this new binding site. Rapid docking methodology was used first to filter the large number of compounds followed by more accurate and slow ones. Fragment based molecular design was adopted afterwards due to unsatisfactory results of screening process. Screening and design process resulted in a group of inhibitors with superior binding energies exceeding that of the natural substrate. Molecular dynamics simulation was used to test the stability of binding of these inhibitors and finally the drug ability of these compounds was assisted using Lipinski rule of five. By this way the designed compounds have shown to possess high pharmacologic potential as novel anticancer agents. |
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Keywords: | VEGFR-2 Docking De novo design Molecular dynamics |
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