| 庚型肝炎病毒—中国河北分离株部分NS3区基因序列分析 |
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| 引用本文： | 徐东刚 牛建章. 庚型肝炎病毒—中国河北分离株部分NS3区基因序列分析[J]. Virologica Sinica, 1998, 13(2): 166-169 |
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| 作者姓名： | 徐东刚 牛建章 |
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| 作者单位： | 军事医学科学院基础医学研究所，河北省卫生防疫站，中国人民解放军３０２医院 |
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| 摘 要： | 在急慢性输血后肝炎、散发性肝炎及爆发型肝炎中，大约１０～２０％的病人不属于已发现的Ａ～Ｅ型肝炎，提示存在新型肝炎病毒。在美国１９９５年第４６届医学年会上，已有了一些有关庚型肝炎病毒（ＨＧＶ）分子生物学及庚型肝炎血清学方面的摘要报道。１９９６年初Ｌｉｎ...
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| 关 键 词： | 庚型肝炎病毒，NS3基因，序列分析 |
Cloning NS3 gene of Hepatitis G Virus from Chinese Patients of Post Transfusion Hepatitis and Its Sequence Analysis |
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| Xu Donggang Lu Haoying Niu Jianzhang Han Fenglian Gao Xin. Cloning NS3 gene of Hepatitis G Virus from Chinese Patients of Post Transfusion Hepatitis and Its Sequence Analysis[J]. 中国病毒学(英文版), 1998, 13(2): 166-169 |
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| Authors: | Xu Donggang Lu Haoying Niu Jianzhang Han Fenglian Gao Xin |
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| Affiliation: | Xu Donggang 1 Lu Haoying 1 Niu Jianzhang 2 Han Fenglian 3 Gao Xin 1 1 |
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| Abstract: | Epitope of HGV NS3 protein was analyzed by Goldkey Program,and according to predicted results,primers of NS3 gene were synthesized.HGV RNA was extracted from patients of post transfusion hepatitis,cDNA fragment of NS3 was amplified by RT PCR.Target gene was cloned into pGEM T vector,and sequence analysis showed that the fragment of NS3 is 591bp,G C is 58.37%.The homology of nucleotide and reduced amino acid between the isolate and other strains from America and China is 84.26%-86.13%,94.42%-94.92%,respectively.The homology between Chinese strains is higher than that between American. |
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| Keywords: | Hepatitis G virus NS3 gene Sequence analysis |
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