1992—1994年HIV—1国际流行株和云南株膜蛋白V3区氨基酸共有序列比较

对１９９２～１９９４年间１６个云南ＨＩＶ１株膜蛋白基因Ｖ３区进行了ＤＮＡ序列测定，经计算机ＤＮＡＳＩＳ及ＰＲＯＳＩＳ软件进行同源性分析，得出其相应的氨基酸共有序列ＹＮＶ３和两组共有序列ＹＮＶ３Ａ和ＹＮＶ３Ｂ，计算了ＹＮＶ３中每个氨基酸的保守性。分别将ＹＮＶ３Ａ和ＹＮＶ３Ｂ与世界各地的ＨＩＶ１代表株的相应序列进行了同源性比较。结果表明，ＨＩＶ１云南株膜蛋白Ｖ３区氨基酸共有序列ＹＮＶ３中每个氨基酸的平均变异度为７．６６％。两组共有序列ＹＮＶ３Ａ和ＹＮＶ３Ｂ，分别与ＨＩＶ１美欧株及泰国流行株Ｂ亚群相应序列有较高同源性。这一结果提示，在进化上云南瑞丽ＨＩＶ１流行毒株间有非常密切的关系，在这一时期该地区的流行毒株以ＨＩＶ１美欧株、泰国株Ｂ亚群及其衍生株为主。

Comparison of Amino Acid Consensus sequences of Membrane Protein V3 Region between International and Local Epidemic HIV 1 Strains from Yunnan During 1992-1994

The amino acid consensus sequences of membrane protein V3 region of HIV 1 Yunnan epidemic strain named YNV3 and two groups of amino acid consensus sequence YNV3A and YNV3B were deduced from DNA sequences of env gene V3 region of 16 local strains collected during 1992-1994,and analysed by DNASIS and PROSIS software. The conservaty of each amino acid in the YNV3 was calculated. The amino acid sequences of YNV3A and YNV3B were compared respectively with that of epidemic strains from other parts of the world for the homology analysis. The results showed that average variation of amino acid was 7.66% in the 35 amino acids which composed the consensus sequences of YNV3. The amino acid consensus sequences of YNV3A and YNV3B showed higher homology with that of HIV 1 American European strain and B subtype of Thailand strain, respectively. The results revealed that there is a closed relation ship between Yunnan Ruili HIV 1 epidemic strains in evolution. The domain epidemic strains in the area were mainly from HIV 1 American European strain, B subtype of Thailand strain and their derivative strains.