人类疱疹病毒6型感染细胞免疫学特性研究

采用间接免疫荧光法、ＡＰＡＡＰ法及ＭＴＴ法，研究了人类疱疹病毒６型（ＨＨＶ６）中国南京地方株ＣＮ５感染细胞病毒抗原表达的形态学和动力学特征、ＣＤ抗原表达阳性细胞百分率的变化及ＰＨＡ诱导的细胞增殖反应的改变。结果显示，ＣＮ５感染脐血单个核细胞（ＣＢＭＣｓ）后８～１２ｈ即可在细胞内检出病毒抗原，至接种后４８ｈ，病毒抗原阳性细胞可达３６％；ＣＮ５感染ＣＢＭＣｓ和成人外周血单个核细胞（ＰＢＭＣｓ）后可引起两者ＣＤ３阳性细胞减少、ＣＤ４阳性细胞增多，而对ＣＤ２、ＣＤ８、ＣＤ４５ＲＡ阳性细胞百分率未见明显影响；ＣＮ５感染细胞裂解液对ＰＨＡ诱导的ＰＢＭＣｓ增殖反应具有抑制作用，这种抑制作用与该裂解液的蛋白浓度之间呈一剂量依赖关系，且可被ＨＨＶ６抗血清所逆转。

Studies on Some Immunological Properties of HHV 6 Infected Cells

The local strain of human herpesvirus 6 (HHV 6),named CN5, was used to study some immunological properties of infected cells. By the methods of indirect immunofluorescence test, APAAP and MTT, the morphological and kinetic character of viral antigen expression, the changes of CD antigens expressions and the cellular proliferation reaction induced by PHA were detected. The viral antigens were appeared in cord blood mononuclear cells (CBMCs) 8-12 hours after infection. By 48 hours positive cells reached 36%. After CN5 infection, in both cases of CBMCs and adult peripheral blood mononuclear cells (PBMCs), the CD3 positive cells decreased while the CD4 positive cells increased. No significant changes were found in the percentages of CD2, CD8 and CD45 RA positive cells. CN5 infected cell lysates inhibited the proliferation of PBMCs induced by PHA in a protein concentration dependent pattern, and this inhibition could be partially eradicated by the antisera to HHV 6.