Proline metabolism and NAD kinase activity in soybean calli during short- and long-term exposures to light and NaCl |
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Authors: | G Guerrier O Delumeau M Renard D Laval-Martin |
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Institution: | (1) Biologie des Ligneux (Associé INRA) EA 1207, Université d'Orléans, BP 6759, 45067 Orléans Cedex 2, France;(2) Groupe de Biochimie et de Biologie Moléculaire Végétales, EA 917, Ensemble Scientifique, 2 Bd Lavoisier, 49045 Angers Cedex, France |
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Abstract: | Calli of soybean (Glycine max Merr.) cv. Maple Arrow grew better and accumulated more proline when cultured for 5 d on 70
mM NaCl under darkness than at light. This rapid proline accumulation in salinized soybean calli appeared to play a protective
role rather than to be a cause of growth failure. Throughout a 28 d-culture cycle (in control and NaCl-treated calli exposed
to light or darkness), we followed the possible relationships between the proline contents and the activities of enzymes of
proline biosynthesis ornithine transaminase; NAD(P)H-pyrroline-5-carboxylate reductase], of proline catabolism NAD(P) proline
dehydrogenase], and of NAD kinase responsible of variations in NADP(H) contents. Enzyme activities of proline metabolism and
NAD kinase were clearly light- and NaCl-regulated; nevertheless, relationships between enzyme activities and proline content
existed only in calli grown for short-term under darkness and in presence of NaCl. The ornithine transaminase route, which
was particularly enhanced in these calli during the first days of salt application, seemed to be involved in the initial proline
accumulation in soybean.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | proline accumulation Glycine max proline biosynthesis proline catabolism salt stress |
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