Regulation of neural progenitor cell state by ephrin-B |
| |
Authors: | Qiu Runxiang Wang Xiuyun Davy Alice Wu Chen Murai Kiyohito Zhang Heying Flanagan John G Soriano Philippe Lu Qiang |
| |
Affiliation: | Division of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA. |
| |
Abstract: | Maintaining a balance between self-renewal and differentiation in neural progenitor cells during development is important to ensure that correct numbers of neural cells are generated. We report that the ephrin-B-PDZ-RGS3 signaling pathway functions to regulate this balance in the developing mammalian cerebral cortex. During cortical neurogenesis, expression of ephrin-B1 and PDZ-RGS3 is specifically seen in progenitor cells and is turned off at the onset of neuronal differentiation. Persistent expression of ephrin-B1 and PDZ-RGS3 prevents differentiation of neural progenitor cells. Blocking RGS-mediated ephrin-B1 signaling in progenitor cells through RNA interference or expression of dominant-negative mutants results in differentiation. Genetic knockout of ephrin-B1 causes early cell cycle exit and leads to a concomitant loss of neural progenitor cells. Our results indicate that ephrin-B function is critical for the maintenance of the neural progenitor cell state and that this role of ephrin-B is mediated by PDZ-RGS3, likely via interacting with the noncanonical G protein signaling pathway, which is essential in neural progenitor asymmetrical cell division. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|