| 重构型人caspase-8基因的表达诱导HeLa细胞凋亡 |
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| 引用本文： | 桂俊豪,赵晶,许彦鸣,于翠娟,贾林涛,王成济,杨安钢.重构型人caspase-8基因的表达诱导HeLa细胞凋亡[J].生物化学与生物物理进展,2003,30(5):738-743. |
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| 作者姓名： | 桂俊豪 赵晶 许彦鸣 于翠娟 贾林涛 王成济 杨安钢 |
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| 作者单位： | 1. 兰州军区乌鲁木齐总医院临床医学研究所,乌鲁木齐,830000
2. 第四军医大学生物化学与分子生物学教研室,西安,710033 |
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| 基金项目： | 教育部留学回国人员科研启动基金（HG99003）和国家杰出青年科学基金（39925036）资助项目. |
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| 摘 要： | 以两种大、小亚基基因次序颠倒的重构型人caspase-8基因的pIRES2-EGFP真核表达载体转染HeLa细胞，用间接免疫荧光染色、免疫细胞化学染色和电镜观察等方法，研究重构型人caspase-8基因在HeLa细胞中的表达及其促凋亡活性.结果显示，两种重构型人caspase-8基因在HeLa细胞中的表达可以有效地诱导HeLa细胞凋亡.
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| 关 键 词： | 重构型，人caspase-8基因，pIRES2-EGFP，表达，HeLa细胞，凋亡 |
| 收稿时间： | 3/2/2003 12:00:00 AM |
| 修稿时间： | 2003年3月2日 |
The Expression and Pro-apoptotic Effects of Reconstructed Human Caspase-8 Genes on HeLa Cells |
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| GUI Jun-Hao,ZHAO Jing,XU Yan-Ming,YU Cui-Juan,JIA Lin-Tao,WANG Cheng-Ji and YANG An-Gang.The Expression and Pro-apoptotic Effects of Reconstructed Human Caspase-8 Genes on HeLa Cells[J].Progress In Biochemistry and Biophysics,2003,30(5):738-743. |
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| Authors: | GUI Jun-Hao ZHAO Jing XU Yan-Ming YU Cui-Juan JIA Lin-Tao WANG Cheng-Ji and YANG An-Gang |
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| Institution: | Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710033, China;Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710033, China;Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710033, China;Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710033, China;Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710033, China;Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710033, China;Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710033, China |
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| Abstract: | The pIRES2-EGFP eukaryotic expression vectors carrying two kinds of reconstructed human caspase-8 genes with rearrangement subunits were transfected into HeLa cells. The expression and pro-apoptotic effects of reconstructed human caspase-8 gene on HeLa cells were analyzed by indirect fluorescent staining, immunohistochemical staining and electronic microscope etc.The results show that expression of the two human caspase-8 can induce HeLa cells apoptosis effectively. |
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| Keywords: | reconstructed human caspase-8 genes pIRES2-EGFP expression HeLa cell apoptosis |
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