Membrane-potential changes in vacuoles isolated from storage roots of red beet (Beta vulgaris L.) |
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Authors: | Anthony J Miller John J Brimelow Philip John |
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Institution: | (1) Department of Agricultural Botany, Plant Science Laboratories, University of Reading, RG6 2AS Reading, UK |
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Abstract: | The membrane potential in vacuoles isolated from storage roots of red beet (Beta vulgaris L.) has been studied by following changes in the fluorescence of the dye 3,3-diethylthiodicarbocyanine iodide, and by determining the uptake of the lipophilic triphenylmethylphosphonium cation. The vacuoles have a membrane potential, internal negative, which is estimated to be around-60 mV. These potentials become less negative by nearly 10 mV on addition of ATP. This ATP-dependent depolarisation is inhibited by the protonophore carbonylcyanide p-trifluoromethoxyphenylhydrazone and by the ATPase inhibitors, N,N-dicyclohexylcarbodiimide and trimethyltin chloride, but it is largely insensitive to sodium orthovanadate. Fusicoccin had no significant effect on the isolated vacuoles, but its addition to excised tissue caused a hyperpolarisation of the cells measured using a microelectrode.Abbreviations DCCD
N,N-dicyclohexylcarbodiimide
- DiS-C2-(5)
3,3-diethylthiodicarbocyanine iodide
- FCCP
carbonylcyanide p-trifluoromethoxyphenylhydrazone
- TPMP+
triphenylmethylphosphonium ion |
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Keywords: | ATPase Beta (membrane potential) Membrane potential Fusicoccin Vacuole (membrane potential) |
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