MIF induces osteoclast differentiation and contributes to progression of periodontal disease in mice |
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Authors: | Madeira Mila Fernandes Moreira Queiroz Celso Martins Costa Graciela Mitre Santos Patrícia Campi Silveira Elcia Maria Garlet Gustavo Pompermaier Cisalpino Patrícia Silva Teixeira Mauro Martins Silva Tarcília Aparecida Souza Daniele da Glória |
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Affiliation: | Department of Microbiology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. |
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Abstract: | Periodontal disease (PD) is a chronic inflammatory and alveolar bone destructive disease triggered by microorganisms from the oral biofilm. Oral inoculation of mice with the periodontopathogen Aggregatibacter actinomycetemcomitans (Aa) induces marked alveolar bone loss and local production of inflammatory mediators, including Macrophage Migration Inhibitory Factor (MIF). The role of MIF for alveolar bone resorption during PD is not known. In the present study, experimental PD was induced in BALB/c wild-type mice (WT) and MIF knockout mice (MIF?/?) through oral inoculation of Aa. Despite enhanced number of bacteria, MIF?/? mice had reduced infiltration of TRAP-positive cells and reduced alveolar bone loss. This was associated with decreased neutrophil accumulation and increased levels of IL-10 in periodontal tissues. TNF-α production was similar in both groups. In vitro, LPS from Aa enhanced osteoclastic activity in a MIF-dependent manner. In conclusion, MIF has role in controlling bacterial growth in the context of PD but contributes more significantly to the progression of bone loss during PD by directly affecting differentiation and activity of osteoclasts. |
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