| UV-B辐射对拟南芥细胞周期G1/S期转变的影响 |
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| 引用本文： | 王静,蒋磊,王艳,李韶山.UV-B辐射对拟南芥细胞周期G1/S期转变的影响[J].植物学报,2009,44(4):426-433. |
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| 作者姓名： | 王静 蒋磊 王艳 李韶山 |
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| 作者单位： | 1 华南师范大学生命科学学院, 广东省高等学校生态与环境科学重点实验室, 广州 510631 2 暨南大学理工学院, 广州 510632 |
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| 基金项目： | 国家自然科学基金,高等学校博士学科点专项科研基金,教育部留学同国人员科研启动基金 |
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| 摘 要： | 以同步化的拟南芥(Arabidopsis thaliana)根尖细胞为材料, 研究了UV-B辐射对拟南芥细胞周期G1/S期转变的影响。细胞周期荧光显微图像分析表明, UV-B辐射延缓了拟南芥根尖细胞G1/S期的转变。基因表达的RT-PCR检测表明, 在UV-B辐射下G1/S期转变的标志基因Histone H4和E2Fa的表达受抑制, 而G1/S期转变的抑制因子KRP2基因表达则受诱导上调。单细胞凝胶电泳检测结果表明, UV-B辐射引起拟南芥根尖细胞内积累大量的环丁烷嘧啶二聚体。以上结果表明, UV-B辐射抑制植物的生长可能受细胞周期和DNA损伤调控。
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| 关 键 词： | 拟南芥 细胞周期 基因表达 G1/S期转变 根尖 |
| 收稿时间： | 2008-11-13 |
| 修稿时间： | 2009-02-06 |
UV-B Induced G1/S Arrest in Arabidopsis Root Tips |
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| Jing Wang,Lei Jiang,Yan Wang,Shaoshan Li.UV-B Induced G1/S Arrest in Arabidopsis Root Tips[J].Bulletin of Botany,2009,44(4):426-433. |
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| Authors: | Jing Wang Lei Jiang Yan Wang Shaoshan Li |
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| Institution: | 1Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Sci ences, South China Normal University, Guangzhou 510631, China 2College of Science and Engineering, Jinan University, Guangzhou 510632, China |
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| Abstract: | Cell cycle regulation is closely linked to plant growth and development. The plant cell cycle responds rapidly to stimuli from both the internal and external environment. We present a model of partially synchronized root tips of Arabidopsis to study the effects of UV-B radiation on the G1/S transition in the cell cycle. Analysis of the fluorescence image under micros copy indicated that G1/S arrest was induc ed by UV-B radiation. The expression of two factors of G1/S transition, Histone H4 and E2Fa, was repressed under UV-B radiation, whereas the negative factor of G1/S transition, KRP2, was upregulated by UV-B radiation. UV-B radiation during the cell cycle induced accumulation of cyclobutane pyrimidine dimers. All these results suggested that UV-B induced plant growth inhibition may be mediated by cell cycle regulation and DNA damage. |
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| Keywords: | , |
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