Homogeneous enzyme immunoassay of diosgenin and its glycosides |
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Authors: | M Sarkar S B Mahato |
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Affiliation: | Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Jadavpur, Calcutta - 700 032, India |
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Abstract: | Homogeneous enzyme immunoassay has been used as a tool for the determination of diosgenin and its glycosides in plants. Diosgenin antisera was found to inhibit the activity of diosgenin hemisuccinate-horseradish peroxidase conjugate which was reversed by the addition of free diosgenin or its glycosides. The increase of enzyme activity was proportional to the quantity of the hapten over a certain range of hapten concentration. Thus, a minimum of 2.5 micrograms/ml of diosgenin and 11.5 micrograms/ml of diosgenin glycosides could be determined by this method. The results were comparable with those obtained by high-performance liquid chromatography and gravimetric methods. |
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Keywords: | enzyme immunoassay of diosgenin steroidal sapogenin diosgenin glycosides |
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