|
|||||
|
|
An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate |
|
| Authors: | Teotia S Khare S K Gupta M N |
| Institution: | a Chemistry Department, Indian Institute of Technology, Hauz Khas, New Delhi 110016, India |
| Abstract: | β-amylases are used in production of maltose syrup. It is shown that sweet potato β-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band and a subunit weight of 50 kDa. Preliminary data with soybean and barley enzymes indicate that this may be a general method for purification of β-amylases. |
| Keywords: | Sweet potato β-Amylase Affinity precipitation Alginate Enzyme purification |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |