Procedures for the automated analyses of proteoglycans and glycosaminoglycans |
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| Authors: | J D Ford J R Baker |
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| Institution: | 1. The Institute of Dental Research, School of Dentistry, University of Alabama in Birmingham, Birmingham, Alabama 35294 USA;2. Department of Medicine, University of Alabama in Birmingham, Birmingham, Alabama 35294 USA |
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| Abstract: | Methods for the automated analysis of hexose, uronic acid, and protein using the Technicon AutoAnalyzer II have been developed by modifying previously published procedures. A method of separating glucosamine and galactosamine, which is eminently suited to quantitating one in the presence of a large amount of the other, is reported. Procedures that can be recommended for determining the amino acid content and individual neutral sugars of proteoglycans or glycosaminoglycans are also described. |
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