Peroxidase and hydrogen peroxide detection by a bioenergized method |
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| Authors: | S M De Toledo M Haun E J Bechara N Durán |
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| Affiliation: | 1. Instituto de Química, Universidade Estadual de Campinas, São Paulo, Brazil;2. Instituto de Biologia, Universidade Estadual de Campinas, São Paulo, Brazil;3. Instituto de Química, Universidade de São Paulo, C.P. 20780, São Paulo, Brazil |
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| Abstract: | Peroxidase activity and hydrogen peroxide concentrations were measured by a chemiluminescent method based upon the reduction of peroxidase Compounds I and II by both eosin and EDTA. Eosin excess present in the reaction mixture was excited during the reaction to its fluorescent state. This bioenergized method allows calculation of peroxidase concentration in the range of 10?12 to 10?7m, and hydrogen peroxide concentration in the range of 10?9 to 10?5m. This approach has been applied to the estimation of peroxidase activity in human red cell membranes and hydrogen peroxide formation in the peroxidase-catalyzed oxidation of glutathione. |
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| Keywords: | To whom inquiries should be addressed. |
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