Computer-controlled maintenance of the dissolved oxygen level in a medium during controlled cultivation of Neisseria meningitidis

A new method for controlling the processes of N. meningitidis cultivation by the content of oxygen dissolved in the medium with the use of a computer, including the software, technical and methodological provision of the computer-operated control system, has been developed. The processes of the cultivation of N. meningitidis, groups A and B, under fully controlled conditions have been realized. The computer-operated control of the content of oxygen in the medium, the most important parameter of the process, has been shown to be essentially more effective than manual control. Information, accumulated in the course of the experiment, may be used in future for correction of mathematical models and optimization of the process. This will by necessary for obtaining biomass with predetermined properties in the development of new diagnostic and vaccine preparations.