Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes |
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Authors: | Wandong Fu Jianting Yao Xiuliang Wang Fuli Liu Zhuo Chong Delin Duan |
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Affiliation: | (1) Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, People’s Republic of China;(2) Graduate School, Chinese Academy of Sciences, Beijing, 100039, People’s Republic of China |
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Abstract: | Extracting DNA from a variety of algae is rather difficult because of high levels of polysaccharides, tannins, and phenolics as these interfere with DNA isolation and downstream applications. High-quality plastid DNA (ptDNA) purification is particularly difficult because of its small proportion in total genomic DNA. This report describes an improved protocol for ptDNA purification that efficiently produces high-quality ptDNA from sporophytes of Laminaria japonica and several other algae. This improved protocol simplifies procedures for ptDNA purification and improves yield to 150–200 μg of ptDNA per 100 g of frozen algal tissue. Polymerase chain reaction (PCR) amplification of conserved sequences has been used to verify purity of the ptDNA product. |
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Keywords: | Brown alga Laminaria japonica Plastid DNA Ultracentrifugation |
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