Glycosylation profiles of airway epithelium after repair of mechanical injury in guinea pigs |
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Authors: | Xiantang L Dorscheid D R Wojcik K R White S R |
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Institution: | (1) Department of Pathology and Committee on Comparative Medicine and Pathology, The University of Chicago, Chicago, IL 60637, USA;(2) Present address: Drug Safety Program, Pfizer, Inc., Eastern Point Road, Groton, CT 06340, USA;(3) Section of Pulmonary and Critical Care Medicine, Department of Medicine, The University of Chicago, Chicago, IL 60637, USA |
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Abstract: | Glycosylated structures on the cell surface have a role in cell adhesion, migration, and proliferation. Repair of the airway epithelium after injury requires each of these processes, but the expression of cell surface glycosylation of airway epithelial cells after injury is not known. We examined cell surface glycosylation using lectin-binding profiles of normal and repairing epithelia in Hartley guinea pigs from 0 to 14 days after mechanical injury. The epithelium regenerated completely over 7 days. In normal trachea, galactose- or galactosamine-specific lectins (14 of 20 tested) labelled epithelial cells, but fucose, mannose, and other sugar-specific lectins (15 tested) did not. GSA-2, a glucosamine-specific lectin, labelled epithelial cells weakly in uninjured tracheas, but intense labelling was noted in basal and non-ciliated columnar cells adjacent to the injury site over 3h to 14 days after injury. Labelling of these cells peaked at 12h and 5 days after injury respectively. Similar patterns were seen with lectins AlloA and HAA but not with CPA during repair. The binding of the lectin DSA to proteins collected from primary cultures of airway epithelial cells decreased substantially after treatment for 24h with either transforming growth factor- or interleukin-1, but that of the CPA lectin did not. We demonstrate changes in glycosylation profiles of airway epithelial cells coordinate with repair after mechanical injury. These changes may be useful to study mechanisms by which repair is regulated. |
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