Production of recombinant human osteoprotegrin from Trichoplusia ni cells and Bombyx mori larvae |
| |
Authors: | Liu Zhen Yang Guan-Zhen Wu Xiang-Fu |
| |
Affiliation: | State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry And Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China. |
| |
Abstract: | Human osteoprotegrin (OPG) and its truncated mutant OPG-280 and lengthened mutant OPG-Fc were constructed and successfully expressed in Trichoplusia ni cells and Bombyx mori larvae. Native SDS-PAGE and Western blot analysis revealed that OPG-Fc is present as a homodimer in Tn cells or B. mori larvae compared with OPG and OPG-280. Furthermore, the hypocalcemic effect assay showed that truncation of the C-terminal 100 residues OPG does not abolish the biological activity and Fc can be helpful in forming the OPG homodimer with improved biological activity. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|