Identification,isolation, and characterization of a human T cell population by a monoclonal antibody |
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Authors: | Richard A Goldsby Barbara A Osborne Edgar G Engleman |
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Institution: | (1) Department of Chemistry, University of Maryland, 20742 College Park, Maryland, USA;(2) National Institutes of Health, 20014 Bethesda, Maryland, USA;(3) Department of Pathology, Stanford University Medical Center, 94305 Stanford, California, USA |
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Abstract: | The hybridization of spleen cells from mice immunized with mononuclear leukocytes with the HAT-sensitive nonsecreting myeloma,
NS1, resulted in the production of hybrid cell lines secreting monoclonal antibodies to lymphocyte surface antigens. One of
these, anti-Ta, was shown by fluorescence-activated cell sorter analysis to be specific for a subpopulation of peripheral
human T cells. Anti-Ta did not react with peripheral human B cells. Immunoprecipitation followed by two-dimensional gel analysis
demonstrated that the T cell subpopulation-specific antigen recognized by this monoclonal antibody is part of, or firmly associated
with, a protein of the plasma membrane. |
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Keywords: | |
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