Transcellular conversion of endogenous arachidonic acid to lipoxins in mixed human platelet-granulocyte suspensions |
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| Authors: | C Edenius J Haeggstr?m J A Lindgren |
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| Institution: | Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden. |
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| Abstract: | Incubation of mixed human platelet/granulocyte suspensions with ionophore A23187 led to a platelet dependent formation of several lipoxin isomers from endogenous substrate. The major metabolite coeluted with authentic lipoxin A4 (5(S), 6(R), 15(S)-trihydroxy-7,9,13-trans-11-cis-eicosatetraenoic acid) in several HPLC-systems and showed an identical UV-spectrum. Furthermore, a similar profile of lipoxins was formed in pure platelet suspensions incubated with exogenous leukotriene A4 (5(S) -5, 6-oxido-7,9-trans-11,14-cis-eicosatetraenoic acid). The conversion of exogenous leukotriene A4 to lipoxin A4 was markedly increased in the presence of ionophore A23187. |
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