| 蔓花生组织培养和植株再生的条件优化 |
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| 引用本文： | 张振霞 郑玉忠. 蔓花生组织培养和植株再生的条件优化[J]. 广西植物, 2009, 29(1): 83-86 |
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| 作者姓名： | 张振霞 郑玉忠 |
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| 作者单位： | 韩山师范学院,生物系,广东,潮州,521041;韩山师范学院,生物系,广东,潮州,521041 |
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| 基金项目： | 国家星火计划，韩山师范学院青年基金 |
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| 摘 要： | 应用MS为基本培养基,通过各种培养条件和不同激素配比,探讨蔓花生组织培养及其植株再生条件的优化。结果显示:幼叶为最佳的外植体,在幼叶愈伤诱导过程中,不超过12h光照,光照强度在21.6μmol.m-2.s-1均可;诱导愈伤组织的适宜培养基为MS+0.5mg/L6-BA+0.2mg/L2,4-D或MS+0.5mg/L6-BA+2mg/LNAA;最适的分化培养基为MS+1mg/LTDZ+2mg/L6-BA+0.5mg/LNAA;最适的生根培养基1/2MS+1mg/LNAA+1mg/LPP333。
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| 关 键 词： | 蔓花生 组织培养 愈伤组织 |
Condition optimization for tissue culture of Arachis duranensis |
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| ZHANG Zhen-Xi,ZHENG Yu-Zhong. Condition optimization for tissue culture of Arachis duranensis[J]. Guihaia, 2009, 29(1): 83-86 |
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| Authors: | ZHANG Zhen-Xi ZHENG Yu-Zhong |
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| Affiliation: | Department of Biology, Hanshan Teachers College, Chaozhou 521041, China |
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| Abstract: | Condition optimization for tissue culture and regeneration of Arachis duranensis was studied.The results showed that the leaflet was the best experimental explant;the optimized medium for callus was MS+0.5 mg/L6-BA+0.2 mg/L2,4-D or MS+0.5 mg/L6-BA+0.2 mg/LNAA with illumination of 12h/d;the optimized medium for differentiation was MS+1 mg/LTDZ+2 mg/L6-BA+0.5 mg/LNAA;the optimized medium for root induction was 1/2MS+1 mg/LNAA+1 mg/LPP333. |
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| Keywords: | Arachis duranensis tissue culture callus |
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