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Purification of the nitrogenase proteins from Clostridium pasteurianum |
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| Authors: | Man-Yin W Tso Torbj rn Ljones and R H Burris |
| Institution: | Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisc. 53706, U.S.A. |
| Abstract: | A method is described for the purification of the nitrogenase proteins from Clostridium pasteurianum by two polyethylene glycol precipitations and chromatography on columns of DEAE-cellulose, Sephadex G-100 and Sephadex G-200. The Mo-Fe protein and the Fe protein have been purified 70–80-fold from the crude extract, and they appear essentially pure when tested by anaerobic polyacrylamide gel electrophoresis. |
| Keywords: | MES 2-(N-morpholino)ethanesulfonic acid |
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