Specific activation of cyclic AMP-dependent protein kinase(s) by H2-histamine agonists in isolated gastric mucosal cells from guinea-pig |
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| Authors: | P. Mangeat G. Marchis-Mouren A.M. Cheret M.J.M. Lewin |
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| Affiliation: | 1. Institut de Chimie Biologique, Université d''Aix-Marseille, C.N.R.S., L.A. 202, Place Victor-Hugo, 13331 Marseille Cédex 3 France;2. I.N.S.E.R.M., U. 10, Hôpital Bichat, 75877 Paris Cédex 18 France |
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| Abstract: | Histamine stimulated cyclic AMP-dependent protein kinase activity in dispersed mucosal cells from guinea-pig gastric fundus (Ka = 5 μM). The H2-agonists dimaprit and impromidine produced similar effects, while the H1-agonist 2-(2-pyridyl) ethylamine had only a weak one. The H2-antagonist cimetidine competitively inhibited 0.1 mM histamine stimulation (Ki = 2 μM). In contrast, the H1-antagonist diphenhydramine had no effect up to 1 mM. |
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| Keywords: | cyclic AMP Protein kinase activation (Guinea-pig mucosa) IBMX 3-isobutyl methylxanthine |
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