Respiration of 14CO2 by intact animals of various species given l-[1-14C]fucose or d-[1-14C]arabinose |
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Authors: | Robert P. Metzger Kathleen D. Edwards Cecilia C. Nixon Patrick W. Mobley |
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Affiliation: | 1. Department of Natural Science, San Diego State University, San Diego, CA 92182, USA;2. Department of Chemistry, California Polytechnic University, Pomona, CA 91768 U.S.A. |
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Abstract: | The production of 14CO2 from l-[1-14C]fucose and d-[1-14C]arabinose has been studied in five mammalian species.Cats, guinea pigs, mice, and rabbits respired about 22% of the label of l[1-14C]fucose or of d-[1-14C]arabinose within 6 h after intraperitoneal injection of the sugar. Rats respired only 1.5% of the l-fucose label and 5% of the d-arabinose label in the same time period.Liver homogenates from cat, guinea pig, and rabbit produced significantly more 14CO2 from l-[1-14C]fucose or d-[1-14C]arabinose than mouse or rat liver homogenates. Unlike those of the other species, guinea pig liver homogenates had very low l-fucose dehydrogenase activity.The results suggest that substantial catabolism of l-fucose and d-arabinose occurs in the tissues of some animal species. Investigators wishing to employ l-fucose as a tracer of glycoprotein metabolism must, therefore, ensure that the species that they employ does not metabolize l-fucose to products interfering with their studies. |
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Keywords: | Carbohydrate metabolism (Mammal) |
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