Intracellular distribution of NADP-linked isocitrate dehydrogenase,fumarase and citrate synthase in bovine heart muscle |
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Authors: | Hasmukh R Fatania Keith Dalziel |
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Institution: | Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU U.K. |
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Abstract: | By fractional extraction of minced bovine heart muscle with iso-osmotic sucrose and phosphate buffer solutions, it is shown that less than 4% of the total citrate synthase in the tissue is in the cytosol. Using citrate synthase as a marker for broken mitochondria, two methods of fractionation of 750 × g supernatants from homogenates of bovine heart muscle show that 10% of the total fumarase and NADP-linked isocitrate dehydrogenase activities are present in the cytoplasm. Homogenates prepared by sonication and osmotic shock and by sand-grinding gave closely similar results as regards enzyme distributions and extent of mitochondrial breakage. The results are compared with those reported for other tissues. |
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Keywords: | Isocitrate dehydrogenase Fumarase Citrate synthase Intracellular distribution NADP (Bovine heart muscle) |
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