β-Hydroxy-α-ketobutyric acid in Drosophila melanogaster,with reference to biosynthesis of drosopterins |
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Authors: | Masahiro Masada Miki Akino |
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Affiliation: | Department of Biology, Tokyo Metropolitan University, Fukazawa Setagaya-ku, Tokyo 158, Japan |
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Abstract: | A substance designated as compound D, which reacts spontaneously with 7,8-dihydropterin to give drosopterins, is found in Drosophila melanogaster. The compound was partially purified from the extract of flies by column chromatography and identified as β-hydroxy-α-ketobutyric acid by analysis of its 2,4-dinitrophenylhydrazone, mass spectrometry and reactivity with 7,8-dihydropterin. A highly significant correlation (r = 0.969, p < 0.001) was found between the amounts of the compound and drosopterins in the eye-pigment mutants of Drosophila. Changes of the compound during development of flies were also closely related to those of drosopterins. Based on these observations, a role of the compound in biosynthesis of drosopterins has been discussed. |
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Keywords: | Drosopterin synthesis Hydroxyketobutyric acid Pterin metabolism (D. melanogaster) PPO diphenyloxazole |
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