Inhibition of collagen breakdown by diphenylhydantoin |
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Authors: | Josef Goultschin Shmuel Shoshan |
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Institution: | 1. Department of Periodontics and Endodontics, Connective Tissue Research Laboratory, Hebrew University Hadassah Faculty of Dental Medicine, Jerusalem, Israel;2. Department of Oral Biology, Connective Tissue Research Laboratory, Hebrew University Hadassah Faculty of Dental Medicine, Jerusalem, Israel |
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Abstract: | Gingival tissue obtained from diphenylhydantoin-treated patients was cultured in the presence of 14C]proline for 24 h. The radioactive medium was removed and the tissue cultured for three days more. DNA, protein, hydroxyproline, proline and radioactivity determinations in the tissue indicated increased cellular proliferation, increased collagen contents and decreased breakdown of collagen in the affected tissues. The media were assayed for dialyzable and non-dialyzable hydroxyproline contents. It was found that the media in which diphenylhydantoin tissues were cultured contained more than twice as much non-dialyzable hydroxyproline than the controls. It was concluded that diphenylhydantoin brought about a reduction in collagen breakdown thus explaining the accumulation of hydroxylated collagen in the tissue. |
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Keywords: | Collagen breakdown Diphenylhydantoin inhibition (Human gingiva) |
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