The influence of initial sucrose and nitrate concentrations on the growth of Cinchona ledgeriana cell suspension cultures and the production of alkloids and anthraquinones |
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| Authors: | Rommert Wijnsma Robert Verpoorte Peter A A Harkes Theo B Van Vliet Hens J G Ten Hoopen Anders Baerheim Svendsen |
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| Institution: | (1) Section of Pharmacognosy, Center for Bio-Pharmaceutical Sciences, Leiden University, Gorlaeus Laboratories, PO Box 9502, 2300 RA Leiden, The Netherlands;(2) Department of Plant Molecular Biology, Subfaculty of Biology, Leiden University, Nonnensteeg 3, 2311 VJ Leiden, The Netherlands;(3) Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands |
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| Abstract: | The influence of initial concentrations of two of the major medium components, sucrose and nitrate, on the growth and the production of alkaloids and anthraquinones in cell suspension cultures of Cinchona ledgeriana Moens was studied. It was found that maximum growth and maximum alkaloid yield were obtained with a B5-medium containing the normal level of nitrate and 4% sucrose, whereas the anthraquinone yield was maximum at 8% sucrose at the normal level of nitrate. Maximum contents of secondary metabolites, expressed as  g.gDW-1, were found using a medium containing 2% sucrose, but four times the normal nitrate concentration. |
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| Keywords: | Cinchona ledgeriana cell culture growth alkaloids anthraquinones medium composition |
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