Plasmid maintenance in Bacillus stearothermophilus is strain-dependent |
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Authors: | Linda Oskam Gerard Venema Sierd Bron |
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Affiliation: | Department of Genetics, Centre of Biological Sciences, Haren, Netherlands. |
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Abstract: | We studied the segregational stability of plasmids based on pTB913, a 4.5-kb rolling-circle plasmid derived from the thermophilic Bacillus plasmid pTB19. In Bacillus stearothermophilus the stability of pTB913 derivatives appeared to be strain-dependent. In strain CU21 large amounts of single-stranded pTB913 DNA were found and the plasmid was highly unstable at 57 degrees C. In strain NUB3621, however, very low amounts of single-stranded plasmid DNA were formed and pTB913-based replicons were only slightly unstable at 57 degrees C. The NUB3621/pTB913 host-vector system seems appropriate for molecular cloning. A RepA-based replicon, also derived from pTB19 but replicating by a theta mechanism, was highly unstable in B. stearothermophilus NUB3621. |
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Keywords: | Thermophilic Bacillus Segregational plasmid stability Rolling-circle replication pTB19 pTB913 Bacillus stearothermophilus |
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