Efficacy of CIM 1166, a combination of compounds derived from Mentha spp. in alleviating experimental vulvovaginal candidiasis in mice |
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Authors: | Dhawan Sangeeta Pal Anirban Ancha Radhika Bawankule Dnyaneshwar Umrao Yadav Narayan Prasad Darokar Mahendra Pandurang Khanuja Suman Preet Singh |
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Institution: | (1) DST Fellow, Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants, Lucknow, India;(2) Bioprospection Group, Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O.CIMAP, Lucknow, 226015, India;(3) Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O.CIMAP, Lucknow, 226015, India |
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Abstract: | Candida albicans is yeast that is most often associated with serious fungal infections and can cause fungal diseases in immuno-compromised
patients especially patients suffering from AIDS, cancer and cases of organ transplant. Amongst women, candidal vaginitis
is predominantly caused by strains of Candida albicans and also remains to be a common problem in immuno-competent or healthy women. A study was undertaken to assess the efficacy
of a compound CIM 1166 obtained from plant source which was found to possess promising antimicrobial property under in vitro
conditions especially against C. albicans. Taking the lead further, a small animal model utilizing aged Swiss albino females that had parturated at least three times
were taken up for model development. Infection (7 × 106 cfu/ml) was instilled into the vagina in a volume of 20 μl for 3 days. Vaginal washings were aseptically collected on day
4th to confirm the establishment of infection following which the treatment was started which continued for the next 5 days
through vaginal route. Vaginal washings were collected on 6th day and the colony forming units were enumerated on chloramphenicol
incorporated SDA plates. The results indicated that there was a significant decrease in the colony forming units in vaginal
washings (8.0 × 102 cfu/ml) of the treated animals as compared to blank control group (6.0 × 104 cfu/ml). The positive control group administered with clotrimazole also showed a recovery from infection with a fungal load
of 8.78 × 102 cfu/ml. The study proves the efficacy of CIM 1166 in curing vaginal candidiasis in mice, which can be taken up for formulation
development and further studies. |
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Keywords: | Vulvovaginal candidiasis Mice Menthol and Menthyl acetate Candida albicans |
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