| Abstract: | Prevalence of Helicobacter is mostly unknown in laboratory animals in
Thailand. The 221 mice feces/cecum from 8 universities, 2 pharmaceutical companies and 3
research institutions in Thailand were surveyed for the prevalence and distribution of
Helicobacter species by using the Electrochemical DNA chip.
Helicobacter were detected 23/46 samples in Specific Pathogen Free
(SPF) and 168/175 in conventional condition. Prevalence of Helicobacter
were 98%, 96%, 92% and 78% in South (n=40), Northeast (n=40), North (n=25) and Central
area (n=116), respectively. Only Central area holds SPF facility resulting in
Helicobacter prevalence that seems to be lower than other areas. Three
species of Helicobacter were detected in feces/cecum samples by sequence
analysis: H. rodentium (67.0%, 148 samples),
Helicobacter sp. MIT 01-6451 (15.4%, 34 samples), and unidentified
Helicobacter species (14.1%, 9 samples). The results suggested that
H. rodentium is the most common species of
Helicobacter in laboratory mice in Thailand. |
